Du leitest Projekte in der Zelllinienentwicklung und optimierst Sequenzen mit Bioinformatik-Tools.
Anforderungen
- •First professional experience of 1-5 years
- •NGS data analysis skills
- •Capacity to implement new methods
- •Programming for automation bioinformatics tools
- •Good command of English
Deine Aufgaben
- •Leite Projekte in der Zelllinienentwicklung
- •Führe Sequenzoptimierung mit Bioinformatik-Tools durch
- •Klonen von Plasmiden und Vorbereitung von Expressionsvektoren
- •Reinige DNA-Fragmente für die Elektroporation
- •Extrahiere genomische DNA und bereite cDNA vor
- •Führe PCR und Reinigung von PCR-Produkten für Sanger-Sequenzierung durch
- •Bereite Platten für NGS-Sequenzierung vor
- •Analysiere Sanger- und NGS-Sequenzierungen
- •Führe qPCR-Analysen durch
- •Führe ddPCR zur Kopienzahl-Analyse durch
- •Teste Mycoplasmen durch PCR
- •Verfasse SOPs und Formulare
- •Erstelle Dokumentationen und Analyseberichte
- •Setze neue Methoden im Molekularbiologielabor um
- •Nehme an F&E-Projekten teil oder führe sie durch
- •Präsentiere interne Projekte
Original Beschreibung
# Molecular Biology Engineer
**Switzerland - Plan-les-Ouates - STELLAR – Route de la Galaise 36** | **Full time**
**Introduction**
Willing to join one an innovative leader in the field of cell line development? Selexis (Geneva, Switzerland) is currently seeking for a talented Molecular Biology Engineer to join our dynamic teams and help the development of recombinant mammalian cell lines. You will play an active role in the expression and characterization of proteins in a cross-functional team environment.
**2. Job title**
Molecular biology engineer
**3. Position overview**
A position is currently open in the department of molecular biology. As part of her/his work, the molecular biology engineer is the dedicated molecular biology operator of a client’s project. He/she will be involved in the project analysis, the design and ordering of optimized nucleotide transgene sequences, the preparation of the corresponding expression vectors, as well as in quality control analyses: verification of a transgene integrated into a cell line by PCR amplification from genomic DNA and amplicon sequencing (Sanger and NGS), mycoplasma testing and assessment of the number of copies integrated in cell lines by ddPCR. The writing of reports as well as the associated documentation are also an important part of his/her task. The implementation of new methods and technologies, as well as the participation in R&D projects constitute another aspect of this position.
**4. General requirements**
Conduct clients project through all the molecular biology steps of cell line development at Selexis. This includes the preparations of DNA and multiple analysis in the lab, as well as in-silico analysis and report writing.
* Sequences optimization including signal peptide screening using bioinformatics tools
* Plasmid cloning and preparation of expression vectors in *E. coli*
* Purification of DNA fragments for electroporation
* Genomic DNA extraction and cDNA preparation
* PCR and purification of PCR products for Sanger Sequencing
* PCR and preparation of the plate for NGS Sequencing
* Sanger and NGS Sequencing analysis
* qPCR (TaqMan and SYBER Green)
* ddPCR (copy number analysis)
* Mycoplasma testing by PCR
* Writing of SOPs and Forms
* Writing of the documentation and analysis reports
* Implementation of new methods / technologies in the molecular biology lab
* Carry out or participate in R&D projects
* Internal project presentation
**5. Preferred qualifications**
* ## Education / Experience
A first professional experience of 1-5 years preferred
## Skills / Knowledge
NGS data analysis skills would be an advantage
Capacity to implement new methods and technologies
Programming for the development of automation bioinformatics tools is a plus
## Languages
A good command of English