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UNUniversitätsklinikum Schleswig-Holstein

PhD position in experimental leukemia research(m/w/x)

Kiel
Full-timeOn-site

Establishing engineered murine leukemia models with retroviral gene transfer and CRISPR/Cas9. Hands-on cell culture and molecular biology experience required. Access to state-of-the-art infrastructure, interdisciplinary environment.

Requirements

  • M.Sc. or equivalent in molecular biology, immunology, biomedical sciences, or related life science discipline
  • Hands-on experience with cell culture and molecular biology methods (e.g., PCR, cloning, transfection)
  • Strong interest to independently acquire in-depth project understanding
  • Willingness to familiarize with novel methods
  • Experience with flow cytometry, primary hematopoietic cell culture, genetic engineering (viral gene transfer/CRISPR) or next-generation sequencing library preparation is advantageous
  • FELASA B equivalent is advantageous for murine transplantation and treatment studies
  • Commitment to acquire animal experiment certificate if no prior experience
  • Focused individual with strong work ethic
  • Ready to engage in cooperative work within the group and with consortia members

Tasks

  • Establish and characterize engineered murine leukemia models
  • Conduct retroviral gene transfer and CRISPR/Cas9 gene editing
  • Validate models using molecular read-outs and flow cytometry
  • Support functional genomics workflows
  • Prepare samples and single-cell libraries for RoCK&ROI-Seq
  • Profile drug responses in high-throughput formats
  • Perform in vitro and in vivo assays
  • Study lineage plasticity and therapy escape
  • Conduct differentiation and co-culture experiments
  • Execute mouse transplantation and immunotherapy experiments
  • Collaborate with CATCH ALL research unit partners

Education

  • Master's degree

Languages

  • EnglishBusiness Fluent

Tools & Technologies

  • PCR
  • cloning
  • transfection
  • flow cytometry
  • primary hematopoietic cell culture
  • genetic engineering
  • viral gene transfer
  • CRISPR
  • next-generation sequencing library preparation

Benefits

Modern Equipment

  • State-of-the-art infrastructure access

Learning & Development

  • Training opportunities

Startup Environment

  • Interdisciplinary environment
  • Supportive environment
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